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Product Details
Synonyms
RP1-261G23.1, MGC70609, MVCD1, VEGFA, VPF
Source
Mouse VEGF164 Protein, Tag Free (VE4-M4216) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 190 (Accession # AAB22253.1 ).
Predicted N-terminus: Ala 27
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 19.3 kDa. The protein migrates as 24-29 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Sterility
Negative
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse VEGF164 Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Mouse VEGF164 Protein, Tag Free (Cat. No. VE4-M4216) was greater than 95% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Mouse VEGF164 Protein, Tag Free (Cat. No. VE4-M4216) at 2 μg/mL (100 μL/well) can bind VEGFR1/R2-Fc with a linear range of 0.6-2.5 ng/mL (QC tested).
Protocol
Immobilized Mouse VEGF164 Protein, Tag Free (Cat. No. VE4-M4216) at 5 μg/mL (100 μL/well) can bind Mouse Neuropilin-1, His Tag (Cat. No. NR1-M52H3) with a linear range of 0.005-0.156 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Vascular endothelial growth factor A
- Category：
- Approved Drugs：
55 Details
- Drugs in Clinical Trials：
56 Details
- Highest Development Stage：
Approved
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