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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 43.9 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human / Cynomolgus / Rhesus macaque ROR1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human / Cynomolgus / Rhesus macaque ROR1, His Tag (Cat. No. RO1-H522y) is more than 95% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Immobilized Human / Cynomolgus / Rhesus macaque ROR1, His Tag (Cat. No. RO1-H522y) at 2 μg/mL (100 μL/well) can bind Anti-ROR1 Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (QC tested).
Immobilized Human / Cynomolgus / Rhesus macaque ROR1, His Tag (Cat. No. RO1-H522y) at 1 μg/mL (100 μL/well) can bind Zilovertamab with a linear range of 0.06-1 ng/mL (Routinely tested).
Anti-Human ROR1 mAb captured on CM5 chip via anti-mouse antibodies surface can bind Human / Cynomolgus / Rhesus macaque ROR1, His Tag (Cat. No. RO1-H522y) with an affinity constant of 4.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Anti-Human ROR1 MAb (Mouse IgG1) on AMC Biosensor, can bind Human / Cynomolgus / Rhesus macaque ROR1, His Tag (Cat. No. RO1-H522y) with an affinity constant of 10.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
2e5 of anti-ROR1 CAR-293 cells were stained with 100 μL of 10 μg/mL of Human / Cynomolgus / Rhesus macaque ROR1, His Tag (Cat. No. RO1-H522y) and negative control protein respectively, washed and then followed by PE-anti-His Tag antibody and analyzed with FACS (Routinely tested).
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