Cytokines ActiveMax®

ActiveMax® Human Activin A, Tag Free, low endotoxin (Cat. No. ACA-H421b) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

ActiveMax® Human G-CSF, Tag Free (Cat. No. GCF-H5214) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

ActiveMax® Human TGFB1, Tag Free (Cat. No. TG1-H4212) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

ActiveMax® Human VEGF165, Tag Free (MALS verified) (Cat. No. VE5-H4210) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%. The purity is more than 95% in HP-SEC, and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.

The bioactivity of ActiveMax® Human GM-CSF, Tag Free (Cat. No. GMF-H4214) was determined by dose-dependent stimulation of the proliferation of TF-1 cells. The ED50 < 0.1 ng/mL, corresponding to a specific activity of > 1x10⁷ Unit/mg.

The bioactivity of ActiveMax® Human IFN-gamma, Tag Free (Cat. No. IFG-H4211) was determined by dose-dependent inhibition of the proliferation of HT-29 cells. The EC50 for this effect is typically 0.41-0.74 ng/mL.

ActiveMax Human VEGF165, Tag Free (MALS verified) (Cat. No. VE5-H4210) stimulates proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 4.216-9.281 ng/mL (Routinely tested).

ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.007-0.014 ng/mL.

Bioactivity of three different lots of ActiveMax® Recombinant Human LIF (Cat. No. LIF-H521b) is verified by ELISA, and the result shows very high batch-to-batch consistency.

Bioactivity of three different lots of ActiveMax® Recombinant Human VEGF165 (Cat. No. VE5-H4210) is verified by cell-based assay, and the result shows very high batch-to-batch consistency.

Bioactivity of three different lots of ActiveMax® Recombinant Human TGF-Beta 1 TGFB1 (Cat. No. TG1-H4212) is verified by cell-based assay, and the result shows very high batch-to-batch consistency.

Bioactivity of three different lots of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) is verified by FACS, and the result shows very high batch-to-batch consistency, and there is no significant difference in activity compared with that of company R.