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| Assay Type | Sandwich-ELISA |
| Analyte | AAV8 |
| Format | 96T |
| Regulatory Status | RUO |
| Sensitivity | <1.75E+07capsids/mL |
| Standard Curve Range | 5.60E+08 capsids/mL-1.75E+07 capsids/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the detection and quantitative determination of AAV8 capsid titration in AAV gene therapy product preparation processing. |
| Sample volume | 100 uL |
| ID | Components | Size |
| AAV08-C01 | Pre-coated Anti-AAV8 Antibody Microplate | 1 plate |
| AAV08-C02 | AAV8 Standard | 1.4E+10 capsids |
| AAV08-C03 | Biotin-Anti-AAV8 Antibody | 15 μg |
| AAV08-C04 | Streptavidin-HRP | 50 μL |
| AAV08-C05 | 10×Washing Buffer | 50 mL |
| AAV08-C06 | 2×Dilution Buffer | 50 mL |
| AAV08-C07 | Substrate Solution | 12 mL |
| AAV08-C08 | Stop Solution | 7 mL |
AAV8 Titration ELISA Kit was developed for the detection and quantitative determination of AAV8 capsid titration in AAV gene therapy product preparation processing.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the AAV8 capsid as standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-AAV8 Antibody diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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