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AAV8 Titration ELISA Kit (Fast)

For research use only.

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Product Details
Assay TypeSandwich-ELISA
AnalyteAAV8
Format96T
Regulatory StatusRUO
Sensitivity<1.94E+08capsids/mL
Standard Curve Range6.22E+09 capsids/mL-1.94E+08 capsids/mL
Assay Time2hr 20 min
Suitable Sample TypeFor the detection and quantitative determination of AAV8 capsid titration in AAV gene therapy product preparation processing.
Sample volume100 uL
Materials Provided
IDComponentsSize
AAV08H-C01Pre-coated Anti-AAV8 Antibody Microplate1 plate
AAV08H-C02AAV8 Standard1.4E+10 capsids
AAV08H-C03HRP-Anti-AAV8 Antibody15 μg
AAV08H-C0410×Washing Buffer 50 mL
AAV08H-C052×Dilution Buffer50 mL
AAV08H-C06Substrate Solution12 mL
AAV08H-C07Stop Solution7 mL
  • Background
    Adeno-associated virus (AAV) has become one of the most important gene vectors in the field of gene therapy due to its long-term expression, low toxicity, low immunogenicity, and high tissue specificity.Most successful AAV gene therapies for preclinical and clinical studies are limited to natural serotypes, but the presence of neutralizing antibodies against AAV remains a significant barrier to systemic delivery.
  • Application

    AAV8 Titration ELISA Kit(Fast) was developed for the detection and quantitative determination of AAV8 capsid titration in AAV gene therapy product preparation processing.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of AAV8 capsid. The kit consists of Pre-coated Anti-AAV8 Antibody Microplate and AAV8 Standard, HRP-Anti-AAV8 Antibody and buffers.

    Your experiment will include 5 simple steps:

    a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

    b) Add your sample to the plate, take the AAV8 capsid as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    c) Add the HRP-Anti-AAV8 Antibody diluted by Dilution Buffer to the plate.

    d) Wash the plate and add TMB.

    e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data Please refer to DS document for the assay protocol.
 AAV8 TYPICAL DATA

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

  • Background: AAV8
    The adeno-associated virus (AAV) is a small (25 nm), non-enveloped virus of the parvoviridae family, including 12 different AAV serotypes. In the parvoviridae family it belongs to the genus dependoparvovirus, because it needs the presence of a helper virus for replication and assembly. The icosahedral AAV capsid composed of the capsid proteins VP1, VP2 and VP3 contains a linear, single-stranded DNA genome of 4.7 kb.
  • Clinical and Translational Updates

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