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| Assay Type | Sandwich-ELISA |
| Analyte | AAV8 |
| Format | 96T |
| Regulatory Status | RUO |
| Sensitivity | <1.94E+08 capsids/mL |
| Standard Curve Range | 6.22E+09 capsids/mL-1.94E+08 capsids/mL |
| Assay Time | 2 hr 20 min |
| Suitable Sample Type | For the detection and quantitative determination of AAV8 capsid titration in AAV gene therapy product preparation processing. |
| Sample volume | 100 μL |
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| AAV08H-C01 | Pre-coated Anti-AAV8 Antibody Microplate | 1 plate |
| AAV08H-C02 | AAV8 Standard | 1.4E+10 capsids |
| AAV08H-C03 | HRP-Anti-AAV8 Antibody | 15 μg |
| AAV08H-C04 | 10×Washing Buffer | 50 mL |
| AAV08H-C05 | 2×Dilution Buffer | 50 mL |
| AAV08H-C06 | Substrate Solution | 12 mL |
| AAV08H-C07 | Stop Solution | 7 mL |
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
To assess the linearity of the assay, samples spiked with high concentrations of AAV8 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
Three AAV8 with different concentrations were tested to calculate the recovery rate.
The specific binding of AAV-A008H to AAV1/2/3/5/6/8/9/dj serotypes was detected. The results showed that AAV-A008H only binds specifically to AAV8.
The specific binding of AAV-A008H to native/denatured AAV8 was detected. The results showed that AAV-A008H only binds specifically to native AAV8. (Denatured AAV8: 95℃ for 10min)
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