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> Cytokines for Organoid Culture
Sterile
Animal-origin Free
High Purity≥95%
High Bioactivity Verified by Organoid Models
Low Endotoxin≤0.1 EU/μg
Carrier Free
Similar to Natural Conformation and Modifications
Consistent between Batches
Gastric organoid
Human EGF (Cat. No. EGF-H52H3), Noggin (Cat. No. NON-H5257), R-spondin1 actively support gastric organoid growth during multiple passages and long-term culture processes.
Liver ductal organoid
Human EGF (Cat. No. EGF-H52H3), Noggin (Cat. No. NON-H5257), R-spondin1 (Cat. No. RS6-H4220), FGF7 (Cat. No. FG7-H52H5), FGF10 , HGF (Cat. No. HGF-H52H3) actively support liver ductal organoid growth.
Brain organoids
BDNF (Cat. No. BDF-H5219), GDNF (Cat. No. GDF-H5219) support iPSC derived human brain organoids formation, expressing neuron marker TUJ1 (green) and neuron stem cell marker NESTIN (red).
Blood vessel organoids
VEGF110 (Cat. No. VE0-H5212) supports iPSC derived blood vessel organoids formation with net-works.
The EGFR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of Human EGF Protein, His Tag, premium grade ( Cat. No. EGF-H52H3). The max induction fold was approximately 56 (Routinely tested).
Human R-Spondin 1 (21-146), His Tag, premium grade (Cat. No. RS6-H4220) induced TCF reporter activity in HEK293 cells. The EC50 for this effect is 0.0138-0.0163 µg/mL (Routinely tested).
Human FGF basic (Cat. No. BFF-H4117) stimulates proliferation of HUVEC in the range of 0-20 ng/mL. The EC50 for this effect is 0.416-0.630 ng/mL (Routinely tested).
The bio-activity of Human Activin A (Cat. No. ACA-H421b) was determined by dose-dependent inhibition of the proliferation of MPC-11 cells. The EC50 for this effect is typically ≤ 9.5 ng/mL (Routinely tested).
Human BMP-2, Tag Free(Cat. No. BM2-H4117) induces alkaline phosphatase production in ATDC5 cells. The EC50 for this effect is 1684-1736 ng/ml (Routinely tested).
The bio-activity of Human PDGF-BB Protein (Cat. No. PDB-H4112) was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. The ED50 was 0.5-20 ng/mL (Routinely tested).
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