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> Réactifs pour construire votre propre analyse immunologique par chimiluminescence à particules magnétiques (MPCLIA)
L'analyse immunologique par chimiluminescence à particules magnétiques (MP) est une méthode d'analyse qui combine la flexibilité des billes magnétiques avec la sensibilité et la spécificité de l'essai CLIA. Au lieu d'immobiliser des protéines ou des anticorps dans la plaque à puits, les billes magnétiques permettent d'augmenter considérablement la surface de contact avec l'échantillon, l'efficacité de la capture et les capacités d'automatisation. Ainsi, la MPCLIA se voit plus fréquemment adopté pour le diagnostic des maladies, la surveillance des réactions chimiques et de nombreux autres domaines de recherche.
Outre l'utilisation de billes magnétiques comme support de base, la principale différence entre la MPCLIA et le test ELISA traditionnel est la détection par chimiluminescence. L'utilisation d'une réaction chimique pour exciter des substances telles que la peroxydase de raifort (HRP) et l'ester d'acridinium (AE), permet de libérer des photons qui peuvent être mesurés à l'aide d'un lecteur de chimiluminescence dédié. En comparaison, les méthodes CLIA ont souvent montré une sensibilité, une spécificité et des plages linéaires supérieures aux méthodes ELISA traditionnelles.
Chemiluminescence Detection Mechanism
Colorimetric (ELISA) Detection Mechanism
| Type de produit | Cat. N° | Description du produit | Pré-commande/Commande |
|---|
| Numéro de l'analyse | Billes magnétiques | Échantillon 1 | Échantillon 2 | Marqueur | Solution de substrat |
|---|---|---|---|---|---|
| 1 | Protein A-coupled Magnetic Beads (Cat. No. MPC-A001) | Human Antibody | Biotinylated non-Fc tag protein | Streptavidin-Acridine ester (Cat. No. STN-NA114) | Substrate Solution(AE Marker) (Cat. No. ABK-001) |
| 2 | hFc tag protein | ||||
| 3 | Protein G-coupled Magnetic Beads (Cat. No. MPC-A002) | Human/Mouse Antibody | Biotinylated non-Fc tag protein | ||
| 4 | Fc tag protein | ||||
| 5 | Anti-His-coupled Magnetic Beads (Cat. No. MPC-A005) | His tag protein | Biotinylated non-His tag protein | ||
| 6 | Human antibody | Anti-Human IgG-Acridine ester (Cat. No. AHG-Y69) | |||
| 7 | hFc tag protein | ||||
| 8 | Anti-Mouse IgG-coupled Magnetic Beads (Cat. No. MPC-A003) | Mouse antibody | Biotinylated non-mFc tag protein | Streptavidin-Acridine ester (Cat. No. STN-NA114) | |
| 9 | mFc tag protein | ||||
| 10* | Mouse antibody | hFc tag protein | Anti-Human IgG-Acridine ester (Cat. No. AHG-Y69) | ||
| 11* | mFc tag protein | Human antibody | |||
| 12* | hFc tag protein | ||||
| 13 | Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006) | Biotinylated non-hFc tag protein | Human antibody | ||
| 14 | hFc tag protein | ||||
| 15 | Biotinylated non-mFc tag protein | Mouse antibody | Anti-Mouse IgG-Acridine ester (Cat. No. AMG-S163) | ||
| 16 | mFc tag protein | ||||
| 17 | Anti-Human IgG-coupled Magnetic Beads (Cat. No. MPC-A004) | Human antibody | mFc tag protein | ||
| 18 | hFc tag protein | ||||
| 19* | hFc tag protein | Mouse antibody | |||
| 20* | Human antibody | Biotinylated non-hFc tag protein | Streptavidin-Acridine ester (Cat. No. STN-NA114) | ||
| 21* | hFc tag protein |
Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg /Test), incubated with 100 μL /Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Protein A-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A001) (10 μg beads/Test). Detection was performed with sensitivity of 3.125 ng/mL in Magnetic Particle Chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
| Cat. No. | Product Description |
|---|---|
| PD1-H82E4 | Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified) |
| STN-NA114 | Streptavidin Protein-Acridine ester (MALS verified) |
| PD1-H5258 | Human PD-L1 / B7-H1 Protein, Fc Tag (MALS verified) |
| MPC-A001 | Protein A-coupled Magnetic Beads (recommended for MPCLIA) |
Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006, 20 μg beads/Test), incubated with 100 μL /Test of Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (Cat. No. PD1-H52A3) at increasing concentration coupled to Anti-Mouse IgG-Acridine ester (Cat. No. AMG-S163, 0.04 μg /Test). Detection was performed with sensitivity of 1.5625 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
| Cat. No. | Product Description |
|---|---|
| PD1-H82E4 | Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified) |
| MPC-A006 | Streptavidin-Magnetic Beads (recommended for MPCLIA) |
| PD1-H52A3 | Human PD-L1 / B7-H1 Protein, Mouse IgG1 Fc Tag, low endotoxin (MALS verified) |
| AMG-S163 | Anti-Mouse IgG-Acridine ester (MALS verified) |
Immobilized 0.04 μg /Test of Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4) to the Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006, 20 μg beads/Test), incubated with 100 μL /Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Anti-Human IgG Antibody-Acridine ester (Cat. No. AHG-Y69, 0.04 μg /Test). Detection was performed with sensitivity of 0.488 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
When used the Chemiluminescent Substrate Solution (AE Marker) (Cat. No. ABK-001) in a sandwich MPCLIA Assay for detection of SARS-CoV-2, The Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (Cat. No. NUN-BM266) coupled Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006) was incubated with Acridine Ester-Labeled Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (AM224) (Cat. No. NUN-M224) and treated with SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5227) at increasing concentration, detection was performed using with sensitivity of 97.7 pg/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
When used the Chemiluminescent Substrate Solution (HRP Marker) (Cat. No. ABK-002) in a sandwich CLEIA Assay for detection of Human IFN-gamma, immobilized Monoclonal Anti-IFNγ antibody, Human IgG1 at 2 μg/mL, add increasing concentrations of Human IFN-gamma, premium grade (Cat. No. IFG-H4211) and then add Biotinylated Biotinylated Monoclonal Anti-IFNγ antibody, Human IgG at 0.05 μg/mL. Detection was performed using Streptavidin-HRP, Horseradish peroxidase conjugated Streptavidin (Cat. No. STN-NH913) with sensitivity of 7.8 pg/mL.
Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg /Test), incubated with 100 μL /Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Protein A-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A001) (10 μg beads/Test). Detection was performed with sensitivity of 3.125 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
The MPCLIA assay shows that Protein G-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A002) is stable at 37°C for 10 days.
The MPCLIA assay shows that Anti-Human IgG-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A004) is stable after freezing and thawing 3 times.
The MPCLIA assay shows that Anti-His Tag-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A005) is stable at 37°C for 10 days.
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