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| ID | Components | Size |
| CRS031-C01 | Pre-coated Anti-Nuclease Antibody Microplate | 1 plate |
| CRS031-C02 | Nuclease Standard | 5 μg |
| CRS031-C03 | Biotin-Anti-Nuclease Antibody | 20 μg |
| CRS031-C04 | Streptavidin-HRP | 50 μL |
| CRS031-C05 | 10×Washing Buffer | 50 mL |
| CRS031-C06 | 2×Dilution Buffer | 50 mL |
| CRS031-C07 | Substrate Solution | 12 mL |
| CRS031-C08 | Stop Solution | 7 mL |
resDetect™ GENIUS™ Nuclease ELISA Kit was developed for the detection and quantitative determination of nuclease in samples from downstream processing where nuclease is used as a process or purification aid.
The limitation of detectable dose (LOD) of Cat. No. CRS-A016 was determined as 2.733 pg/mL.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the Nuclease as standard. The samples and standard are diluted by Dilution Buffer.
c) Add the Biotin-Anti-Nuclease Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
resDetect™ GENIUS™ Nuclease ELISA Kit (Residue Testing) (Cat. No. CRS-A031) can detect the nuclease in different culture supernatant with similar curve and sensitivity.
resDetect™ GENIUS™ Nuclease ELISA Kit (Residue Testing) (Cat. No. CRS-A031) can detect the nuclease from different culture supernatant and was free from the matrix effects.
resDetect™ GENIUS™ Nuclease ELISA Kit (Residue Testing) (Cat. No. CRS-A031) can detect the nuclease from different manufacturers, a nuclease levels differ from manufacturer to manufacturer.
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
Three Nuclease with different concentrations were tested to calculate the recovery rate.
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