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Familles de protéines d'interleukine

Interleukin Protein Families
Introduction

"Interleukines" (IL) est un terme qui décrit un groupe de molécules sécrétées par les leucocytes et qui agissent sur une variété de cellules. En général, les IL sont classées parmi les cytokines, mais elles font également partie de la famille des lymphokines. Les IL ont diverses fonctions biologiques ; elles participent non seulement à la régulation de la réponse immunitaire en favorisant la transmission d'informations, l'activation et la régulation des cellules immunitaires, mais elles sont aussi largement impliquées dans la régulation des processus physiologiques et pathologiques de l'organisme tels que les maladies auto-immunes, inflammatoires et tumorales. Sur la base de l'homologie structurelle des cytokines, les interleukines sont divisées en plusieurs familles de protéines, telles que les familles IL-1, IL-2, IL-6, IL-10, IL-12 et IL-17.

Il convient de noter que la famille des interleukines est devenue une cible importante pour les médicaments, dont beaucoup sont déjà sur le marché et occupent même une part importante du marché des médicaments pour des maladies similaires.

Data from Pharmacodia
Data from Pharmacodia
Data from Pharmacodia
Produits en vogue

Données supplémentaires
Haute pureté et grande homogénéité structurelle vérifiées par SDS-PAGE et SEC-MALS
ILR-H5253

Biotinylated Human IL-1 RAcP, His,Avitag (Cat. No. ILP-H82E5) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 90%. As verified by SEC-MALS, the purity is more than 90% and the molecular weight of this protein is around 50-68 kDa.

IL4-C5259

Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. ILG-H5257) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%. As verified by SEC-MALS, the purity is more than 90% and the molecular weight of this protein is around 160-190 kDa.

Activité biologique élevée vérifiée par ELISA
High purity and structural homogeneity verified by SDS-PAGE and SEC-MALS

Immobilized Mouse IL-2, His Tag (Cat. No. IL2-M52H3) at 5 μg/mL (100 μL/well) can bind Mouse IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. ILG-M5253) with a linear range of 0.8-3 ng/mL (QC tested).


High purity and structural homogeneity verified by SDS-PAGE and SEC-MALS

Immobilized Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-4, Avitag,His Tag (Cat. No. IL4-H82E0) with a linear range of 0.1-2 ng/mL (Routinely tested).

Affinité vérifiée par SPR et BLI
High purity and structural homogeneity verified by SDS-PAGE and SEC-MALS

Human IL-31, His Tag (Cat. No. IL1-H5247) capture on NTA-Series S sensor chip can bind Human IL-31 RA, Fc Tag (Cat. No. ILA-H5256) with an affinity constant of 37.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

High purity and structural homogeneity verified by SDS-PAGE and SEC-MALS

Human IL-13 R alpha 1 Protein, His Tag (Cat. No. IL1-H5224) captured on CM5 chip via anti-His antibody can bind Human IL-13 with an affinity constant of 16 nM as determined in a SPR assay (Biacore T200) (QC tested).

High purity and structural homogeneity verified by SDS-PAGE and SEC-MALS

Loaded Biotinylated Human IL-6, epitope tag free, primary amine labeling (Cat. No. IL6-H8218) on SA Biosensor, can bind Human IL-6 R alpha, His Tag (Cat. No. ILR-H4223) with an affinity constant of 45.9 nM as determined in BLI assay (ForteBio Octet Red96e).

High purity and structural homogeneity verified by SDS-PAGE and SEC-MALS

Loaded Biotinylated Human IL17A, His,Avitag (Cat. No. ILA-H82Q1) on SA Biosensor, can bind Human IL-17 RA, His Tag (Cat. No. ILA-H5222) with an affinity constant of 18.8 nM as determined in BLI assay (ForteBio Octet Red96e).

Références
  • 1. Chung S H, Ye X Q, Iwakura Y. Interleukin-17 family members in health and disease[J]. International immunology, 2021, 33(12): 723-729. https://doi.org/10.1093/intimm/dxab075.
  • 2. Wojno E D T, Hunter C A, Stumhofer J S. The immunobiology of the interleukin-12 family: room for discovery[J]. Immunity, 2019, 50(4): 851-870. https://doi.org/10.1016/j.immuni.2019.03.011.
  • 3. Rose-John S. Interleukin-6 family cytokines[J]. Cold Spring Harbor perspectives in biology, 2018, 10(2): a028415. https://doi.org/10.1101/cshperspect.a028415.
  • 4. Netea M G, van de Veerdonk F L, van der Meer J W M, et al. Inflammasome-independent regulation of IL-1-family cytokines[J]. Annual review of immunology, 2015, 33: 49-77. https://doi.org/10.1146/annurev-immunol-032414-112306.
  • 5. Zdanov A. Structural analysis of cytokines comprising the IL-10 family[J]. Cytokine & growth factor reviews, 2010, 21(5): 325-330.doi:10.1016/j.cytogfr.2010.08.003.
  • 6. Dumont F J. Interleukin-2 family cytokines: potential for therapeutic immmunoregulation[J]. Expert Opinion on Therapeutic Patents, 2005, 15(5): 521-554. https://doi.org/10.1517/13543776.15.5.521.
  • 7. Fickenscher H, Hör S, Küpers H, et al. The interleukin-10 family of cytokines[J]. Trends in immunology, 2002, 23(2): 89-96. https://doi.org/10.1016/S1471-4906(01)02149-4.
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