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Service d'analyse immuno-enzymatique (ELISA)
Les tests immuno-enzymatiques (ELISA) constituent l'une des principales méthodes de détection des analytes depuis des décennies. En tant qu'expérience incontournable de toute recherche sur le développement de médicaments, des données ELISA fiables et de haute qualité sont essentielles pour la compréhension scientifique et les investigations ultérieures.
L'avantage d'ACRO provient de son personnel scientifique qualifié et expérimenté, de son large catalogue de protéines, d'anticorps, y compris les anticorps anti-idiotypes, ainsi que de ses kits ELISA prêts à l'emploi, tous produits en interne.
Nous fournissons un service complet de développement de méthodes de dosage biologique ELISA, qui comprend le développement de méthodes, la validation, le transfert et le développement de kits, en fonction de chacun de vos besoins, afin de vous aider à accélérer le processus de développement de vos médicaments !
ACROBiosystems fournit des mesures d'absorbance, de fluorescence et de luminescence de haute qualité en utilisant le système SpectraMax i3x (Molecular Devices). ACROBiosystems a également acquis l'extension ScanLaterTM Western Blot ainsi que la cartouche de détection Alpha Screen 384 STD, offrant ainsi à nos clients de multiples options d'analyse.
Sensibilité sur tout le spectre avec l'éclairage Spectral Fusion™
Gamme dynamique étendue avec PMT refroidi
Cartouches pour FP, AlphaScreen et Western Imaging
Sur la base du système SpectraMax i3x, ACROBiosystems fournit des services de recherche et développement biomédical aux clients qui cherchent à quantifier et à détecter l'interaction anticorps-antigène. Les autres services comprennent, sans s'y limiter, l'activité anti-drogue (ADA), les tests PK/PD ainsi que le dépistage des anticorps.
Le rapport d'analyse sera livré dans les 2 semaines suivant la réception de l'échantillon !
ACROBiosystems propose une gamme complète de services de développement de méthodes de dosage biologique ELISA, allant des réactifs de dosage à la détection des échantillons !
Service à guichet unique de développement de méthodes de dosage biologique ELISA
We provide screening services including peptide screening and antibody screening using common methods like competitive ELISA.
For example, we can perform a screening assay for peptide samples using ACROBiosystem's own Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Kit (Cat#: RAS-N022).
The microplate in the kit has been pre-coated with Human ACE2 protein. First serum samples, Positive control, Negative Control are added to the wells followed by addition of HRP-SARS-CoV-2 Spike RBD. The presence of neutralizing antibodies in samples will compete with ACE2 for HRP-SARS-CoV-2 Spike RBD binding. The intensity of assay signal decrease proportionally to the presence of Anti-SARS-CoV-2 neutralizing antibody.
Fig1. Positive reference for anti-SARS-CoV-2 neutralizing antibody concentrations (μg/mL) for peptide 9 screening (SpectraMax i3x).
Fig2. Sample screening for peptide 9 (uM) using anti-SARS-CoV-2 neutralizing antibody titer serologic Kit (Cat#: RAS-N022) (SpectraMax i3x).
Fig3. Different anti-BCMA antibodies have different neutralization ability for BCMA and BAFF.
ln addition to antigen affinity screening, affinity of antibody to Fc receptors and C1q is responsible for cell killing via ADCC/ADCP and CDC effects respectively. Therefore testing association of Fc receptors and C1q is necessary during the development phase of antibody drug candidates.
Fig4. Standard curve of mouse complement C3a to determine the concentrations of C3a in client mouse plasma samples (SpectraMax i3X).
We can perform a binding assay for Human TROP-2 and a client’s antibody to test the efficacy of the provided antibody using our own Human TROP-2 / TACSTD2 Protein, His Tag (Cat#: TR2-H5223). The microplate is coated overnight with TROP-2. The sample antibody is added to the wells, and subsequently the secondary HRP conjugated Goat Anti-Mouse IgG is added. After the primary antibody from the client binds to TROP-2 the HRP secondary antibody will bind to the primary, and following the addition of TMB the intensity of the signal will be proportional to the concentration of primary antibody.
Fig5. Binding curve comparing the concentration of anti-TROP-2 antibody (nM) to the optical density to determine the EC50 (SpectraMax i3x).
An idiotope is the unique set of antigenic determinants (epitopes) of the variable portion of an antibody. An anti-idiotypic (Anti-ID) antibody binds to the idiotope of another antibody, usually an antibody drug, which makes it a very powerful tool for antibody drug development, especially for immunogenicity and PK/PD analysis.
To support preclinical/clinical immunogenicity and PK analysis, ACROBiosystems has developed a series of high-affinity anti-idiotypic antibodies and ADA/PK services.
Fig6. Immobilized Adali**mab at 1 µg/ml, add increasing concentrations of anti-Adali**mab antibodies (Cat. No. ADB-Y19, 10% human serum) and then add biotinylated Adali**mab at 5 µg/ml. Detection was performed using HRP-conjugated
ACRO has developed conjugated magnetic beads which can be used in beads based ELISA.
ACROBiosystems scientist will respond within 24 hours of submission.
Call us at: +1 800-810-0816 or email at services@acrobiosystems.com for consultation.
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