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Cynomolgus CD3 epsilon Protein, Fc,His Tag

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  • Synonym
    FLJ18683,T3E,TCRE,CD3E,CD3-epsilon
  • Source
    Cynomolgus CD3 epsilon, Fc,His Tag(CDE-C5254) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (Accession # Q95LI5).
    Predicted N-terminus: Gln 22
  • Molecular Characterization
    CD3 epsilon Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 38.3 kDa. The protein migrates as 43-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD3 epsilon SDS-PAGE

Cynomolgus CD3 epsilon, Fc,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 CD3 epsilon ELISA

Immobilized SP34-2 at 2 μg/mL (100 μL/well) can bind Cynomolgus CD3 epsilon, Fc,His Tag (Cat. No. CDE-C5254) with a linear range of 0.2-3 ng/mL (Routinely tested).

Bioactivity-SPR
 CD3 epsilon SPR

Bispecific T-cell Engager immobilized on CM5 Chip can bind Cynomolgus CD3 epsilon, Fc,His Tag (Cat. No. CDE-C5254) with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €350.00

Price(EUR) : €2200.00

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Drug Development Status

  • Number of Launched Drugs:3 Details
  • Number of Drugs in Clinical Trials:7 Details
  • Latest Research Phase:Approved

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