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Limited Edition Golden Llama is here! Check out how you can get one. 
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Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.7 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 14 kDa (B2M) respectively due to different glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-R82W7) is more than 90% and the molecular weight of this protein is around 53-71 kDa verified by SEC-MALS.
Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-R82W7) captured on Biotin CAP-Series S Sensor Chip can bind Herceptin® with an affinity constant of 10.3 nM as determined in a SPR assay (Biacore T200) (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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