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Your Position: Accueil > Protein > B7-H6 > B76-H82E5

Biotinylated Human B7-H6 / NCR3LG1 Protein, His,Avitag™

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  • Synonym
    B7-H6,NCR3LG1,B7 Homolog 6
  • Source
    Biotinylated Human B7-H6, His,Avitag(B76-H82E5) is expressed from human 293 cells (HEK293). It contains AA Asp 25 - Ser 262 (Accession # Q68D85-1).
    Predicted N-terminus: Asp 25
  • Molecular Characterization
    B7-H6 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 30.4 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
B7-H6 SDS-PAGE

Biotinylated Human B7-H6, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 B7-H6 ELISA

Immobilized Biotinylated Human B7-H6, His,Avitag (Cat. No. B76-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human NKp30, Fc Tag (Cat. No. NC3-H5259) with a linear range of 0.2-2 ng/mL (Routinely tested).

Bioactivity-SPR
 B7-H6 SPR

Biotinylated Human B7-H6, His,Avitag (Cat. No. B76-H82E5) immobilized on SA Chip can bind Human NKp30, His Tag (Cat. No. NC3-H5228) with an affinity constant of 0.213 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Background
    The B7 family of genes is essential in the regulation of the adaptive immune system. one of which is the recently discovered B7H6. Humans and rats have a single B7H6 gene; however, many B7H6 genes were detected in a single large cluster in the Xenopus genome.
    Chimeric antigen receptor (CAR) T-cell therapies have demonstrated durable and potentially curative therapeutic efficacy against B-cell leukemia in clinical trials. In this study, B7H6, a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

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