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Your Position: Accueil > Protein > BTN3A2 > BT2-H82E7

Biotinylated Human BTN3A2 Protein, His,Avitag™

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  • Synonyme
    BT3.2,BT3.3,BTF3,BTF4
  • Source
    Biotinylated Human BTN3A2, His,Avitag (BT2-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Trp 248 (Accession # P78410-1).
    Predicted N-terminus: Gln30
  • Caractérisation moléculaire
    Online(Gln 30 - Trp 248) P78410-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 27.3 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxine
    Less than 1.0 EU per μg by the LAL method.
  • Pureté

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Stockage:

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human BTN3A2, His,Avitag (Cat. No. BT2-H82E7) SDS-PAGE gel

Biotinylated Human BTN3A2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • CONTEXTE
    Butyrophilin subfamily 3 member A2(BTN3A2/BT3.2) mRNA expression by tumoral cells was previously identified as a prognostic factor in a small cohort of high grade serous epithelial ovarian cancer (HG-EOC). We find that the three BTN3A isoforms: BTN3A1, BTN3A2, and BTN3A3, have high structural homology to the B7 superfamily of proteins and exist as V-shaped homodimers in solution, associating through the membrane proximal C-type Ig domain.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(EUR) : €350.00

Price(EUR) : €1390.00

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