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Anti-Adalimumab Antibody (AY19) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
AY19
Mouse
Mouse IgG1/kappa
Hybridoma Monoclonal
Human
Adalimumab.
Recognizes Adalimumab specifically, no cross reactivity with other humanized antibodies.
| Application | Recommended Usage |
| ELISA | 5-600 ng/mL |
>95% as determined by SDS-PAGE.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-Adalimumab Antibody (AY19) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Anti-Adalimumab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development.Immobilized adalimumab at 1 µg/mL, add increasing concentrations of Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19, 10% human serum) and then add biotinylated adalimumab at 5 µg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 0.6 ng/mL (QC tested).
Demonstration of the specificity of Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19) to the adalimumab.
Measured by its neutralizing ability in a functional ELISA. Immobilized adalimumab at 0.5 μg/mL (100 μL/well) can bind pre-mixed Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19) and Biotinylated Human TNF-alpha, His,Avitag (Cat. No. TNA-H82E3) with an inhibition rate of 100%.
Anti-Adalimumab Antibody (AY19) (mouse IgG1, Cat. No. ADB-Y19) captured on CM5 chip via anti-mouse antibodies surface, can bind human adalimumab with an affinity constant of 1.36 pM.
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