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Anti-Cetuximab Antibody (AY27) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
AY27
Mouse
Mouse IgG1/kappa
Hybridoma Monoclonal
Human
Cetuximab.
Recognizes Cetuximab specifically, no cross reactivity with other humanized antibodies.
| Application | Recommended Usage |
| ELISA | 20-2500 ng/mL |
>95% as determined by SDS-PAGE.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-Cetuximab Antibody (AY27) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Anti-Cetuximab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development. Immobilized cetuximab at 1 µg/mL, add increasing concentrations of Anti-Cetuximab Antibody (AY27) (Cat. No. CEB-Y27, 10% human serum) and then add biotinylated cetuximab at 2 µg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 1.2 ng/mL (QC tested).
Demonstration of the specificity of Anti-Cetuximab Antibody (AY27) (Cat. No. CEB-Y27) to the cetuximab.
Measured by its neutrlizing ability in a functional ELISA. Immobilized cetuximab at 0.2 μg/mL (100 μL/well) can bind pre-mixed Anti-Cetuximab Antibody (AY27) (Cat. No. CEB-Y27) and Biotinylated Human EGFRvIII, His,Avitag (Cat. No. EGR-H82E0) with a inhibition rate of 100%.
Anti-Cetuximab Antibody (AY27) (mouse IgG1, Cat. No. CEB-Y27) captured on CM5 chip via anti-mouse antibodies surface, can bind Human cetuximab with an affinity constant of 7.28 pM.
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