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Biotinylated Anti-Cetuximab Antibody (AY31) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
AY31
Mouse
Mouse IgG1 | kappa
Hybridoma Monoclonal
Human
Cetuximab.
Recognizes Cetuximab specifically, no cross reactivity with other humanized antibodies.
>90% as determined by SDS-PAGE.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Anti-Cetuximab Antibody (AY31) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Anti-Cetuximab Antibody (12B1E9) (Cat. No.CEB-Y29c) at 2 μg/mL, add increasing concentrations of Cetuximab and then add Biotinylated Anti-Cetuximab Antibody (AY31) (Cat. No.CEB-BY31) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4.84 ng/mL (QC tested).
Immobilized cetuximab at 0.1 μg/mL (100 μL/well) can bind Biotinylated Anti-Cetuximab Antibody (AY31) (Cat. No. CEB-BY31) with a linear range of 0.39-25 ng/mL.
ELISA analysis shows that the binding of cetuximab to Human EGF R, His Tag (Cat. No. EGR-H5222) was inhibited by increasing concentration of Biotinylated Anti-Cetuximab Antibody (AY31) (Cat. No. CEB-BY31). The concentration of cetuximab used is 3 ng/mL.
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