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Anti-Bevacizumab Antibody (AY10) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
AY10
Mouse
Mouse IgG1/kappa
Hybridoma Monoclonal
Human
Bevacizumab.
Recognizes Bevacizumab specifically, no cross reactivity with other humanized antibodies.
| Application | Recommended Usage |
| ELISA | 10-20000 ng/mL |
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-Bevacizumab Antibody (AY10) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Anti-Bevacizumab Antibody (AY10) (Cat. No. BEB-Y10) is more than 95% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
Detection of bevacizumab by bridging ELISA in serum. Immobilized Anti-Bevacizumab Antibody (AY10) (Cat. No. BEB-Y10) at 2 μg/mL, add increasing concentrations of bevacizumab (10% human serum) and then add Biotinylated Anti-Bevacizumab Antibody (AY13) (Cat. No. BEB-BY13) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 0.4 μg/mL (QC tested).
Immobilized Bevacizumab at 1 μg/mL (100 μL/well) can bind Anti-Bevacizumab Antibody (AY10) (Cat. No. BEB-Y10) with a linear range of 0.39-25 ng/mL.
Immobilized Bevacizumab at 2 μg/mL (100 μL/well) can bind pre-mixed Anti-Bevacizumab Antibody (AY10) (Cat. No. BEB-Y10) and Biotinylated Human VEGF165, His,Avitag (Cat. No. VE5-H82Q0) with a inhibition rate of 81%.
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