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Biotinylated Mouse IgG1 Isotype Control (mAb) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
Mouse
Mouse IgG1 | kappa
Biotin
Recombinant Monoclonal
Mouse
Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.
| Application | Recommended Usage |
| ELISA | 1-150 ng/mL |
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Mouse IgG1 Isotype Control (mAb) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) is more than 95% and the molecular weight of this protein is around 135-170 kDa verified by SEC-MALS.
Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) with a linear range of 1-39 ng/mL (QC tested).
2e5 of Anti-CD19 CAR-293 cells were stained with a series of concentrations of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1), washed and then followed by PE-SA and analyzed with FACS (QC tested).
5e5 of PBMCs were stained with a series of concentrations of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1), washed and then followed by PE-SA and analyzed with FACS (QC tested).
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) and positive control respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
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