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Biotinylated Human CD7 Protein, His,Avitag™, premium grade

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  • Synonym
    CD7,GP40,TP41,LEU-9,Tp40
  • Source
    Biotinylated Human CD7 Protein, His,Avitag, premium grade(CD7-H82E4) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Pro 180 (Accession # P09564-1).
    Predicted N-terminus: Ala 26
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Molecular Characterization
    CD7 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 20.0 kDa. The protein migrates as 28-38 kDa under reducing (R) condition, and 26-36 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Host Cell Protein
    <0.5 ng/µg of protein tested by ELISA.
  • Host Cell DNA
    <0.02 ng/μg of protein tested by qPCR.
  • Sterility
    Negative
  • Mycoplasma
    Negative.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD7 SDS-PAGE

Biotinylated Human CD7 Protein, His,Avitag, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD7 MALS images

The purity of Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) is more than 90% and the molecular weight of this protein is around 52-62 kDa verified by SEC-MALS.

Bioactivity-FACS
 CD7 FACS

2e5 of Anti-CD7 CAR-293 cells were stained with 100 μL of 3 μg/mL of Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) and negative control protein respectively, washed and then followed by PE-Streptavidin and analyzed with FACS (QC tested).

Bioactivity-ELISA
 CD7 ELISA

Immobilized Anti-CD7 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) with a linear range of 0.5-8 ng/mL (QC tested).

 CD7 ELISA

Immobilized Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-CD7 antibody, Mouse IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).

  • Background
    T-cell antigen CD7 (CD7) is also known as GP40, LEU-9, TP41 and Tp40. CD7 is a protein that in humans is encoded by the CD7 gene, this gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. CD7 has been shown to interact with PIK3R1. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €370.00

Price(EUR) : €1610.00

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  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:24 Details
  • Latest Research Phase:Phase 2 Clinical

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