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Limited Edition Golden Llama is here! Check out how you can get one. 
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Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 41.5 kDa (CD3E) and 41.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. As a result of glycosylation, the protein migrates as 48-55 kDa under reducing (R) condition, and 100 kDa under non-reducing (NR) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-M52W2) is more than 90% and the molecular weight of this protein is around 80-95 kDa verified by SEC-MALS.
Immobilized Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-M52W2) at 1 μg/mL (100 μL/well) can bind Biotinylated CD3e Monoclonal Antibody (Clone : 145-2C11) with a linear range of 0.2-6 ng/mL (QC tested).
Immobilized CD3E Monoclonal Antibody (Clone : 145-2C11) at 1 μg/mL (100 μL/well) can bind Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-M52W2) with a linear range of 0.01-0.25 μg/mL (Routinely tested).
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