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Anti-Trastuzumab Antibody (AY1b) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
AY1b
Mouse
Mouse IgG1/kappa
Recombinant Monoclonal
Human
Trastuzumab.
Recognizes Trastuzumab specifically, no cross reactivity with other humanized antibodies.
| Application | Recommended Usage |
| ELISA | 0.39-100 ng/mL |
>95% as determined by SDS-PAGE.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-Trastuzumab Antibody (AY1b) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Trastuzumab at 1 μg/mL (100 μL/well) can bind Anti-Trastuzumab Antibody (AY1b) (Cat. No. TRB-Y1b) with a linear range of 0.4-6 ng/mL (QC tested).
Immobilized Anti-Trastuzumab Antibody (AY1b) (Cat. No. TRB-Y1b) at 2 μg/mL, add increasing concentrations of Trastuzumab in the 10% human serum and then add Biotinylated Anti-Trastuzumab Antibodies at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 48 ng/mL (QC tested).
Serial dilutions of Anti-Trastuzumab Antibody (AY1b) (Cat. No. TRB-Y1b) was detected by Biotinylated Human Her2, His,Avitag (Cat. No. HE2-H82E2) with a half maximal inhibitory concentration (IC50) of 4.58 μg/mL (Routinely tested).
Trastuzumab captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Anti-Trastuzumab Antibody (AY1b) (Cat. No. TRB-Y1b) with an affinity constant of 0.729 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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