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Anti-Rituximab Antibody is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
Mouse
Mouse IgG1/kappa
Recombinant Monoclonal
Human
Rituximab.
Recognizes Rituximab specifically, no cross reactivity with other humanized antibodies.
| Application | Recommended Usage |
| ELISA | 0.2-50 ng/mL |
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-Rituximab Antibody on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Anti-Rituximab Antibody (Cat. No. RIB-Y35c) is more than 90% and the molecular weight of this protein is around 135-155kDa verified by SEC-MALS.
Immobilized Rituximab at 1 μg/mL (100 μL/well) can bind Anti-Rituximab Antibody (Cat. No. RIB-Y35c) with a linear range of 0.2-6 ng/mL (QC tested).
Immobilized Rituximab at 2 μg/mL, add increasing concentrations of Anti-Rituximab Antibody (Cat. No. RIB-Y35c) in the 10% human serum and then add Anti-CD20, Human IgG1 at 5 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 100 ng/mL (QC tested).
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