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Anti-Adalimumab Antibody (AY23b) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
AY23b
Mouse
Mouse IgG1/kappa
Recombinant Monoclonal
Human
Adalimumab.
Recognizes Adalimumab specifically, no cross reactivity with other humanized antibodies.
| Application | Recommended Usage |
| ELISA | 0.2-100 ng/mL |
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-Adalimumab Antibody (AY23b) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Anti-Adalimumab Antibody (AY23b) (Cat. No. ADB-Y23b) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Immobilized Adalimumab at 1 μg/mL (100 μL/well) can bind Anti-Adalimumab Antibody (AY23b) (Cat. No. ADB-Y23b) with a linear range of 0.2-3 ng/mL (QC tested).
Immobilized Anti-Adalimumab Antibody (AY23b) (Cat. No. ADB-Y23b) at 2 μg/mL, add increasing concentrations of Adaliumumab and then add Biotinylated Biotinylated Anti-Adalimumab Antibody (AY17) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (QC tested).
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