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Limited Edition Golden Llama is here! Check out how you can get one. 
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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 40.2 kDa. The protein migrates as 46-56 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 52 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human R-Spondin 1 (21-146), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human LRP-6 (20-630), Mouse IgG2a Fc Tag (Cat. No. LR6-H5253) at 5 μg/mL (100 μL/well) can bind Human R-Spondin 1 (21-146), Fc Tag (Cat. No. RS1-H5269) with a linear range of 0.008-0.5 μg/mL (QC tested).
Immobilized Human R-Spondin 1 (21-146), Fc Tag (Cat. No. RS1-H5269) at 5 μg/mL (100 μL/well) can bind Human LGR5, His Tag (Cat. No. LG5-H52H3) with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Human R-Spondin 1 (21-146), Fc Tag (Cat. No. RS1-H5269) induced TCF reporter activity in HEK293 cells. The EC50 for this effect is 0.0311 µg/mL (Routinely tested).
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