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Rat CD27 / TNFRSF7 Protein, His Tag

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  • Synonym
    CD27,TNFRSF7,S152,T14,Tp55
  • Source
    Rat CD27, His Tag(CD7-R52H9) is expressed from human 293 cells (HEK293). It contains AA Thr 21- Arg 183 (Accession # NP_001019506.1).
    Predicted N-terminus: Thr 21
  • Molecular Characterization
    CD27 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 19.8 kDa. The protein migrates as 30-35 kDa and 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD27 SDS-PAGE

Rat CD27, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 CD27 ELISA

Immobilized Mouse CD27 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-M5251) at 2 μg/mL (100 μL/well) can bind Rat CD27, His Tag (Cat. No. CD7-R52H9) with a linear range of 2-39 ng/mL (QC tested).

 CD27 ELISA

Immobilized Human CD27 Ligand, Fc Tag (active trimer) (MALS verified) (Cat. No. CDL-H5266) at 2 μg/mL (100 μL/well) can bind Rat CD27, His Tag (Cat. No. CD7-R52H9) with a linear range of 2-78 ng/mL (Routinely tested).

  • Background
    CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €350.00

Price(EUR) : €2330.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:5 Details
  • Latest Research Phase:Phase 2 Clinical

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