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| ID | Components | Size |
| EP146-C01 | High-bind Plate | 1 plate |
| EP146-C02 | Human IL-6 R alpha | 15 μg |
| EP146-C03 | Biotinylated Human IL-6 | 10 μg |
| EP146-C04 | Anti-IL-6 Neutralizing Antibody | 10 μg |
| EP146-C05 | Streptavidin-HRP | 10 μg |
| EP146-C06 | Coating Buffer | 12 mL |
| EP146-C07 | 10xWashing Buffer | 50 mL |
| EP146-C08 | Blocking Buffer | 50 mL |
| EP146-C09 | Substrate Solution | 12 mL |
| EP146-C10 | Stop Solution | 7 mL |
This kit is used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product by various biotechnological processes such as cell or tissue culture.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-6 R alpha.
b) Add your molecule of interest to the tests.
c) Add human IL-6-Biotin to bind the coated IL-6 R alpha.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to IL-6: IL-6 R alpha binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF IL-6 [Biotinylated]: IL-6 R alpha BINDING BY ANTI-IL6 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-IL-6 Neutralizing antibody (Catalog # EP146-C04) (1:1 serial dilution, from 10 μg/mL to 0.01μg/mL) was added into Biotinylated IL-6: IL-6 R alpha binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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