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Your Position: Accueil > Protein > Cas12a > CAA-L5140

GENPower™ NLS-Cas12a Nuclease (MALS verified)

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  • Features

    With two NLS, which leads to transport to the nucleus efficiently.
    High cleavage activity

  • Product Details
    GENPower™ NLS-Cas12a Nuclease is a recombinant Lachnospiraceae bacterium Cas12 protein purified from Escherichia coli for CRISPR-based genome editing. The introduction of nuclear localization signals (NLS) can help Cas12 enter the nucleus, increasing the chance of genomic DNA cleavage.
  • Application

    Developed as a variety of genome editing tools, such as transcription regulation, gene recombination, and base editing.

  • Concentration
    10 μg/μL
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Sterility
    Negative
  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM NaAC, 500 mM NaCl, pH6.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt;
    2. -20°C for 3 months under sterile conditions.
SDS-PAGE
Cas12a SDS-PAGE

The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Cas12a MALS images

The purity of GENPower™ NLS-Cas12a Nuclease (Cat. No. CAA-L5140) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.

Bioactivity
 Cas12a ENZYME

Measured by its ability to cleave a targeted DNA substrate. Cas12a achieves >90% substrate cleavage (QC tested).

  • Clinical and Translational Updates

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