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ID | Components | Size |
EP159-C01 | High-bind Plate | 1 plate |
EP159-C02 | Human IL-11 | 55 μg |
EP159-C03 | Biotinylated Human IL-11R | 20 μg |
EP159-C04 | Anti-IL-11 Neutralizing Antibody | 20 μg |
EP159-C05 | Streptavidin-HRP | 5 μg |
EP159-C06 | Coating Buffer | 12 mL |
EP159-C07 | 10xWashing Buffer | 50 mL |
EP159-C08 | Blocking Buffer | 50 mL |
EP159-C09 | Substrate Solution | 12 mL |
EP159-C10 | Stop Solution | 7 mL |
This kit is developed for screening for inhibitors of human IL-11 binding to human IL-11R.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-11.
b) Add your molecule of interest to the tests.
c) Add human IL-11R-Biotin to bind the coated IL-11.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to IL-11:IL-11R binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF IL-11: IL-11R [Biotinylated] BINDING BY ANTI-IL-11 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-IL-11 Neutralizing antibody (Catalog # EP159-C04) (1:1 serial dilution, from 10 μg/mL to 0.0195μg/mL) was added into IL-11: Biotinylated IL-11R binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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