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The kit is developed for quantitative detection of natural and recombinant human ROR1 in serum, plasma and cell culture supernatants.
It is for research use only.
| Analyte | Soluble ROR1 |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | 0.78125ng/mL |
| Range | 0.78125 ng/mL-200 ng/mL |
| Assay Time | 2hr |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 μL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of ROR1 is less than 0.78125 ng/mL.
Ten replicates of each of 4 samples containing different ROR1 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Five samples containing different concentrations of ROR1 were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant ROR1 was spiked into 3 human serum samples, and then analyzed. The average recovery of ROR1 for serum samples is 103.2%.
| ID | Components | Size |
| CEA050-C01 | Pre-coated Anti-ROR1 Antibody Microplate | 1 plate |
| CEA050-C02 | Human ROR1 Standard | 20 μg×2 |
| CEA050-C03 | Biotin-Anti-ROR1 Antibody Con. Solution | 400 μL |
| CEA050-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA050-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CEA050-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA050-C07 | 20× Washing Buffer | 50 mL |
| CEA050-C08 | Sample Dilution Buffer | 15 mL ×2 |
| CEA050-C09 | Substrate Solution | 12 mL |
| CEA050-C10 | Stop Solution | 6 mL |
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