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Your Position: Accueil > Protein > IGFBP-4 > IG4-H5253

Human IGFBP-4 Protein, Fc Tag (MALS verified)

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  • Synonym
    IGFBP4,IBP-4
  • Source
    Human IGFBP-4 Protein, Fc Tag(IG4-H5253) is expressed from human 293 cells (HEK293). It contains AA Asp 22 - Glu 258 (Accession # P22692-1).
    Predicted N-terminus: Asp 22
  • Molecular Characterization
    IGFBP-4 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 52.4 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IGFBP-4 SDS-PAGE

Human IGFBP-4 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IGFBP-4 MALS images

The purity of Human IGFBP-4 Protein, Fc Tag (Cat. No. IG4-H5253) is more than 85% and the molecular weight of this protein is around 100-125 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IGFBP-4 ELISA

Immobilized Human IGF-I, His Tag (Cat. No. IG1-H5245) at 5 μg/mL (100 μL/well) can bind Human IGFBP-4 Protein, Fc Tag (Cat. No. IG4-H5253) with a linear range of 1-20 ng/mL (QC tested).

 IGFBP-4 ELISA

Immobilized Human IGFBP-4 Protein, Fc Tag (Cat. No. IG4-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IGF-II, Avitag,Fc Tag (Cat. No. IG2-H82F9) with a linear range of 1-20 ng/mL (Routinely tested).

  • Background
    Insulin-like growth factor-binding protein 4 (IGFBP-4) is also known as IGF-binding protein 4, which contains an IGFBP domain and a thyroglobulin type-I domain. IGFBP-4 binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. IGFBP-4 is secreted by all colon cancer cells.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

Price(EUR) : €2330.00

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