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Your Position: Accueil > Protein > Streptavidin > STN-NA117

Streptavidin Protein-ALP, Alkaline Phosphatase conjugated Streptavidin

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  • Synonym
    Streptavidin,SA
  • Source
    Streptavidin is expressed from E. coli cells and conjugated with alkaline phosphatase under optimal conditions.
    Predicted N-terminus: Met
  • Molecular Characterization

    This protein carries no "tag".

    The protein has a calculated MW of 13.8 kDa.

  • Application
    Recommended for use in ALP Chemiluminescence System such as CLEIA (0.1 µg/mL) or MPCLIA, immunohistochemistry, and western blot applications. Avoid using biotin-containing solutions as diluents. It is recommended that the reagent be titrated for optimal performance for each application. NOTE: Do not use skim milk as a blocking agent in the assay with streptavidin, since skim milk contains free biotin which will cause high backgrounds.
  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 3 M NaCl, 1 mM MgCI2, 0.1 mM ZnCI2, pH7.5, 1% BSA, 0.03% Proclin300 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
Bioactivity-CLEIA
 Streptavidin CLEIA

Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M12A1) at 1 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike RBD (L452R, T478K), His,Avitag (Cat. No. SPD-C82Ed) with a linear range of 0.0049-10 ng/mL when detected by Streptavidin Protein-ALP, Alkaline Phosphatase conjugated Streptavidin (0.1 µg/mL) (Cat. No. STN-NA117) (QC tested).

  • Background
    Streptavidin is a 66KDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity to biotin. Each unit can bind one biotin. Horseradish peroxidase is metalloenzyme, a 44KDa glycoprotein. When incubate with substrates, it produces a coloured, fluorimetric, or luminescent derivatives, which can be detected and quantified. HRP conjugated Streptavidin is widely used for the detection and quantification of biotinylated proteins.
  • Clinical and Translational Updates

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Price(EUR) : €70.00

Price(EUR) : €170.00

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