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| Assay Type | Sandwich-ELISA |
| Analyte | DLL4 |
| Format | 96T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | < 18.75 pg/mL |
| Standard Curve Range | 18.75 pg/mL-1200 pg/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the quantitative determination of human DLL4 in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 100 uL |
| ID | Components | Size |
| RES052-C01 | Pre-coated Anti-DLL4 Antibody Microplate | 1 plate |
| RES052-C02 | Human Fc Tag DLL4 Standard | 20 μg |
| RES052-C03 | Biotin-Anti-DLL4 Antibody | 150 μL |
| RES052-C04 | Streptavidin-HRP | 50 μL |
| RES052-C05 | 10xWashing Buffer | 50 mL |
| RES052-C06 | 2xDilution Buffer | 50 mL |
| RES052-C07 | Substrate Solution | 12 mL |
| RES052-C08 | Stop Solution | 7 mL |
Human Fc Tag DLL4 ELISA KIT was developed for the detection and quantitative determination of GMP human Fc Tag DLL4(ACROBiosystems, cat#GMP-GMP-DL4H28) concentration.
It is for research use only.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the Human Fc Tag DLL4 Standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-DLL4 Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
To assess the linearity of the assay, samples spiked with high concentrations of human Fc Tag DLL4 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜10%.
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.
Three parts of blank serum were added with different concentrations of human Fc Tag DLL4, and the serum without human Fc Tag DLL4 was used as background to calculate the recovery rate. The range of the recovery rate is 83.7-116.5%, and the average recovery is 98.6%.
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