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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.9 kDa. The protein migrates as 37-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Fc epsilon RI alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human Fc epsilon RI alpha, His Tag (Cat. No. FCA-H5228) is more than 90% and the molecular weight of this protein is around 37-55 kDa verified by SEC-MALS.
Immobilized Immunoglobulin E, Human Plasma at 5 μg/mL (100 μL/well) can bind Human Fc epsilon RI alpha, His Tag (Cat. No. FCA-H5228) with a linear range of 0.4-3 ng/mL (QC tested).
Loaded Human Fc epsilon RI alpha, His Tag (Cat. No. FCA-H5228) on AR2G Biosensor, can bind Immunoglobulin E, Human Plasma with an affinity constant of 2.71 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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