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| Assay Type | Sandwich-ELISA |
| Analyte | Cas9 |
| Format | 96T(8×12 strips) |
| Reactivity | Streptococcus pyogenes |
| Regulatory Status | RUO |
| Sensitivity | ＜0.781ng/mL |
| Standard Curve Range | 0.781 ng/mL-50 ng/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the quantitative determination of Cas9 in Cell Therapy. |
| Sample volume | 100 uL |
| ID | Components | Size |
| CAS01-C01 | Pre-coated Anti-Cas9 Antibody Microplate | 1 plate(8×12 strips) |
| CAS01-C02 | Cas9 Nuclease Standard | 20 μg |
| CAS01-C03 | Biotin-Anti-Cas9 Antibody | 15 μg |
| CAS01-C04 | Streptavidin-HRP | 50 μL |
| CAS01-C05 | 10xWashing Buffer | 50 mL |
| CAS01-C06 | 2xDilution Buffer | 50 mL |
| CAS01-C07 | Substrate Solution | 12 mL |
| CAS01-C08 | Stop Solution | 7 mL |
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the Cas9 Nuclease Standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-Cas9 Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
To assess the linearity of the assay, samples spiked with high concentrations of Cas9 nuclease were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
Three Cas9 nuclease with different concentrations were tested to calculate the recovery rate.
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