0
Il n'y a pas de produit dans le panier !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Accueil > Protein > IL-18BP > ILP-H82E3

Biotinylated Human IL-18BP Protein, His,Avitag™ (SPR verified)

Order Now

  • Synonym
    IL18BP,IL18BPa,Tadekinig-alfa
  • Source
    Biotinylated Human IL-18BP Protein, His,Avitag(ILP-H82E3) is expressed from human 293 cells (HEK293). It contains AA Thr 31 - Gly 194 (Accession # O95998-2).
    Predicted N-terminus: Thr 31
  • Molecular Characterization
    IL-18BP Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 21.2 kDa. The protein migrates as 33-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-18BP SDS-PAGE

Biotinylated Human IL-18BP Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-SPR
 IL-18BP SPR

Human IL-18 Protein, premium grade (Cat. No. IL8-H5114) immobilized on CM5 Chip can bind Biotinylated Human IL-18BP Protein, His,Avitag (Cat. No. ILP-H82E3) with an affinity constant of 0.184 nM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    Interleukin-18-binding protein (IL18BP) is also known as Tadekinig-alfa, IL18BPa, which functions as an IL18 inhibitor. IL18BP is a secreted protein which contains one Ig-like C2-type (immunoglobulin-like) domain. IL18BP does not have a transmembrane domain and hence is not anchored to the cell membrane. IL18BP is strongly expressed in heart, lung, placenta and spleen. Isoform A of IL18BP binds to IL-18 and inhibits its activity. IL18BP also functions as an inhibitor of the early TH1 cytokine response. IL18BP suppresses the production of IFN-gamma resulting in reduced T-helper type 1 immune responses.
  • Clinical and Translational Updates

Comments (0)


ETA des produits en stock: 2 jours ouvrables

Price(EUR) : €350.00

Price(EUR) : €1390.00

Recommended Products
Plaque enduite de streptavidine

Price(EUR) : €30.00

Price(EUR) : €140.00

Streptavidine-HRP

Price(EUR) : €50.00

Price(EUR) : €120.00

Billes magnétiques™ Streptavidine

Price(EUR) : €140.00

Price(EUR) : €230.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop

Laisser un message