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The kit is developed for quantitative detection of natural and recombinant human FGF basic/FGF2/bFGF in serum, plasma and cell culture supernatants.
It is for research use only.
| Analyte | bFGF/FGF2 |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | 7 pg/ml |
| Range | 10.94 pg/mL-700 pg/mL |
| Assay Time | 2.75 hr |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 uL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of bFGF/FGF2 is less than 7 pg/mL.
Ten replicates of each of 3 samples containing different bFGF/FGF2 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
3 samples containing different concentrations of bFGF/FGF2 were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant bFGF/FGF2 was spiked into 3 human serum samples, and then analyzed. The average recovery of bFGF/FGF2 for serum samples is 100.9%.
| ID | Components | Size |
| CEA082-C01 | Pre-coated Anti-bFGF/FGF2 Antibody Microplate | 1 plate |
| CEA082-C02 | Human bFGF/FGF2 Standard | 20μg ×2 |
| CEA082-C03 | Biotin-Anti-bFGF/FGF2 Antibody Con. Solution | 100 μL |
| CEA082-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA082-C05 | Streptavidin-HRP Con. Solution | 500 uL |
| CEA082-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA082-C07 | 20× Washing Buffer | 50 mL |
| CEA082-C08 | Sample Dilution Buffer | 15 mL ×2 |
| CEA082-C09 | Substrate Solution | 12 mL |
| CEA082-C10 | Stop Solution | 6 mL |
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