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Influenza A virus (Wisconsin/67/2022) NP (H1N1) Protein, His Tag

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  • Source
    Influenza A virus (Wisconsin/67/2022) NP (H1N1) Protein, His Tag (NP1-V52H3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Asn 498 (Accession # EPI2224781, GISAID).
  • Molecular Characterization
    Nucleocapsid protein/NP (Influenza Virus) Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 58.1 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.2 M Arginine, PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Nucleocapsid protein/NP (Influenza Virus) SDS-PAGE

Influenza A virus (Wisconsin/67/2022) NP (H1N1) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 Nucleocapsid protein/NP (Influenza Virus) ELISA

Immobilized Influenza A virus (Wisconsin/67/2022) NP (H1N1) Protein, His Tag (Cat. No. NP1-V52H3) at 1 μg/mL (100 μL/well) can bind Pan Influenza A Nucleoprotein Antibody, Mouse MAb with a linear range of 0.05-1.56 ng/mL (QC tested).

 Nucleocapsid protein/NP (Influenza Virus) ELISA

Immobilized Pan Influenza A Nucleoprotein Antibody, Mouse MAb at 1 μg/mL (100 μL/well) can bind Influenza A virus (Wisconsin/67/2022) NP (H1N1) Protein, His Tag (Cat. No. NP1-V52H3) with a linear range of 1-63 ng/mL (Routinely tested).

Bioactivity-SPR
 Nucleocapsid protein/NP (Influenza Virus) SPR

Pan Influenza A Nucleoprotein Antibody, Mouse MAb immobilized on CM4 Chip can bind Influenza A virus (Wisconsin/67/2022) NP (H1N1) Protein, His Tag (Cat. No. NP1-V52H3) with an affinity constant of 3.32 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Influenza, commonly known as ‘the flu’, is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses.Influenza viral nucleoprotein (NP) is highly conserved and the most abundant non-enzymatic viral protein in infected cells. NP is a key component of the viral ribonucleoproteins (vRNPs) complex, and its recognized functions include, but are not limited to, binding to RNA and oligomerizing for the vRNP complex, undergoing intracellular trafficking, and participating in the switch from mRNA transcription to vRNA replication.
  • Clinical and Translational Updates

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Price(EUR) : €350.00

Price(EUR) : €2710.00

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