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Limited Edition Golden Llama is here! Check out how you can get one. 
Limited Edition Golden Llama is here! Check out how you can get one.
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Time Limited Offer: Welcome Gift for New Customers ! Shipping Price Reduction for EU Regions
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 80.7 kDa. The protein migrates as 90 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human DLL4 Protein, Fc Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human DLL4 Protein, Fc Tag, premium grade (Cat. No. DL4-H5255) is more than 90% and the molecular weight of this protein is around 160-200 kDa verified by SEC-MALS.
Human DLL4 Protein, Fc Tag, premium grade (Cat. No. DL4-H5255) captured on Protein A Chip can bind Human NOTCH1 Protein, His Tag, premium grade (Cat. No. NO1-H52H3) with an affinity constant between 1.00 nM - 150 nM as determined in a SPR assay (Biacore 8K) (QC tested).
CD34+ CD45+ hematopoietic cells were seeded on a Human DLL4 Protein, Fc Tag, premium grade (Cat. No.DL4-H5255) coating plate and differentiated for 14 days, then flow cytometry was used to detect the expression of T-cell progenitor markers, CD7 and CD5, in FSC/SSClow gate. Human DLL4 Protein, Fc Tag, premium grade (Cat. No.DL4-H5255) together with other factors could induce the formation of CD7+ and CD7+ CD5+ T-cell progenitors. However, the cells cultured without Human DLL4 Protein, Fc Tag, premium grade (Cat. No.DL4-H5255) expressed neither CD5 nor CD7 (Routinely tested).
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