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Monoclonal Anti-Human VP0 Antibody, Human IgG1 (1D10) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
1D10
Mouse
Human IgG1 | Human Kappa
Unconjugated
Recombinant Monoclonal
Virus
This product is a specific antibody specifically reacts with VP0.
| Application | Recommended Usage |
| Western Blot | 0.1-10 ug/mL |
| ELISA | 0.05-3.1 ng/mL |
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Monoclonal Anti-Human VP0 Antibody, Human IgG1 (1D10) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Monoclonal Anti-Human VP0 Antibody, Human IgG1 (1D10) (Cat. No. VP0-MY321) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Immobilized Human enterovirus 71 (strain USA/BrCr/1970) (EV71) VP0 Protein, His Tag (Cat. No. VP0-V5244) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human VP0 Antibody, Human IgG1 (1D10) (Cat. No. VP0-MY321) with a linear range of 0.04-0.8 ng/mL (QC tested).
Detection of Monoclonal Anti-Human VP0 Antibody, Human IgG1 (1D10), Human IgG1 | Human Kappa, HEK by Western Blot. Monoclonal Anti-Human VP0 Antibody, Human IgG1 (1D10), Human IgG1 | Human Kappa, HEK at 0.1ug/ml dilution + Human enterovirus 71 (strain USA/BrCr/1970) (EV71) VP0 Protein, His Tag at 400ng.
Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.
Predicted band size: 65 kDa 12% Bis-Tris Protein Gel.
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