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PE-Labeled Human TIGIT Protein, His TagStar Staining

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  • Synonym
    TIGIT,VSIG9,VSTM3
  • Source
    PE-Labeled Human TIGIT Protein, His Tag(TIT-HP2H3) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).
    Predicted N-terminus: Met 22
  • Molecular Characterization
    TIGIT Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 41.4 kDa.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Evaluation of CAR expression
FACS Analysis of TIGIT binding to 293T overexpressing CD155 cells
 TIGIT CAR_T

Flow cytometric analysis of 293T overexpressing CD155 cells staining with PE-Labeled Human TIGIT Protein, His Tag (Cat. No. TIT-HP2H3) at 1:50 dilution (2 μL of the stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) , compared with negative control protein(Fig. B and A). PE signal was used to evaluate the binding activity (QC tested).

  • Background
    T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
  • Clinical and Translational Updates

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