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Mouse BTLA Protein, Fc Tag (MALS verified)

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  • Synonym
    BTLA,CD272
  • Source
    Mouse BTLA, Fc Tag (BTA-M5253) is expressed from human 293 cells (HEK293). It contains AA Glu 30 - Gly 176 (Accession # Q32MV9).
    Predicted N-terminus: Glu 30
  • Molecular Characterization
    Online(Glu 30 - Gly 176) Q32MV9

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 42.8 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse BTLA, Fc Tag (Cat. No. BTA-M5253) SDS-PAGE gel

Mouse BTLA, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Mouse BTLA, Fc Tag (Cat. No. ) MALS images

The purity of Mouse BTLA, Fc Tag (Cat. No. BTA-M5253) is more than 90% and the molecular weight of this protein is around 90-120 kDa verified by SEC-MALS.

  • Background
    B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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