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Limited Edition Golden Llama is here! Check out how you can get one. 
Limited Edition Golden Llama is here! Check out how you can get one.
Offering SPR-BLI Services - Proteins provided for free! Get your ComboX free sample to test now!
Time Limited Offer: Welcome Gift for New Customers ! Shipping Price Reduction for EU Regions
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 43.6 kDa. The protein migrates as 55-70 kDa under reducing (R) condition, and 110-130 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) is more than 90% and the molecular weight of this protein is around 133-163 kDa verified by SEC-MALS.
Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) with a linear range of 5-78 ng/mL (QC tested).
Immobilized Anti-CD28 MAb, Mouse IgG1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) with a linear range of 2.4-78 ng/mL (Routinely tested).
Loaded Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) on SA Biosensor, can bind Human B7-1, Fc Tag (Cat. No. B71-H5259) with an affinity constant of 56.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) on SA Biosensor, can bind Human B7-1, Mouse IgG2a Fc Tag (Cat. No. B71-H52A4) with an affinity constant of 38.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Price(EUR) : €270.00
Price(EUR) : €1150.00
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Price(EUR) : €120.00
Price(EUR) : €140.00
Price(EUR) : €230.00
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