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Limited Edition Golden Llama is here! Check out how you can get one. 
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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 41.6 kDa. The protein migrates as 53-66 kDa under reducing (R) condition, and 100-120 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human / Cynomolgus / Rhesus macaque CD28, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) is more than 90% and the molecular weight of this protein is around 105-125 kDa verified by SEC-MALS.
Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) at 2 μg/mL (100 μL/well) can bind Anti-Mouse CD28 MAb with a linear range of 0.1-2 ng/mL (QC tested).
Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-1, Fc Tag (Cat. No. B71-H82F2) with a linear range of 0.313-5 μg/mL (Routinely tested).
Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) on CM5 Chip can bind Human B7-1, His Tag (Cat. No. B71-H5228) with an affinity constant of 5.29 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) on CM5 Chip can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) with an affinity constant of 2.72 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) on CM5 Chip can bind Human B7-1, Fc Tag (Cat. No. B71-H5259) with an affinity constant of 2.01 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) on ProteinA Biosensor, can bind Human B7-1, His Tag (Cat. No. B71-H5228) with an affinity constant of 2.2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) on Protein A Biosensor, can bind Human B7-2, His Tag (Cat. No. CD6-H5223) with an affinity constant of 6.8 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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