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Limited Edition Golden Llama is here! Check out how you can get one. 
Limited Edition Golden Llama is here! Check out how you can get one.
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This protein carries a llama IgG2b Fc tag at the C-terminus
The protein has a calculated MW of 34.4 kDa. The protein migrates as 42-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human BAFFR Protein, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human BAFF, His Tag at 2 μg/mL (100 μL/well) can bind Human BAFFR, Llama IgG2b Fc Tag (Cat. No. BAR-H5258) with a linear range of 2-125 ng/mL (QC tested).
Immobilized Human BAFFR Protein, Llama IgG2b Fc Tag (Cat. No. BAR-H5258) at 2 μg/mL (100 μL/well) can bind Biotinylated Human BAFF, His,Avitag (Cat. No. BAF-H82Q2) with a linear range of 0.2-4 ng/mL (Routinely tested).
Immobilized Human BAFFR Protein, Llama IgG2b Fc Tag (Cat. No. BAR-H5258) at 1 μg/mL (100 μL/well) can bind Anti-BAFFR Antibody, Human IgG1 with a linear range of 0.2-5 ng/mL (QC tested).
Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR Protein, Llama IgG2b Fc Tag (Cat. No. BAR-H5258): Biotinylated Human BAFF Protein, His,Avitag (Cat. No. BAF-H82Q2) binding reactions. The half maximal inhibitory concentration (IC50) is 1.1323 μg/mL (Routinely tested).
Immobilized Human BAFF Protein, Fc Tag (Cat. No. BAF-H5261) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human BAFFR Protein, Llama IgG2b Fc Tag (Cat. No. BAR-H5258) with an affinity constant of 44.5 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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