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Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
Y45
Mouse
Mouse IgG1 | Mouse Kappa
Unconjugated
Recombinant Monoclonal
Human
FMC63.
Specifically recognizes the antigen-recognition domain of FMC63 derived antibody.
| Application | Recommended Usage |
| ELISA | 6.25-50 ng/mL |
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1) is more than 95% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.
Immobilized FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1) with a linear range of 0.1-6 ng/mL. FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) is verified not recoginized by Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) in low concentration (QC tested).
ELISA analysis shows that the binding of Human CD19, Fc Tag (Cat. No. CD9-H5251) to FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) was inhibited by increasing concentration of Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1). The concentration of Human CD19, Fc Tag used is 5 μg/mL (100 μL/well). The IC50 is 0.084 μg/mL (Routinely tested).
Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1) captured on CM5 chip via anti-mouse antibodies surface can bind FMC63 scFv with an affinity constant of 1.08 nM as determined in a SPR assay (Routinely tested).
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