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Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with PE-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG1 (1G10) (Cat. No. FM3-PFM721) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).
Non-specificity of PE-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG1 (1G10) (Cat. No. FM3-PFM721) binding to CD3+ cells present in human PBMC. Human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and PE-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG1 (1G10) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL), washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (Routinely tested).
Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1) captured on CM5 chip via anti-mouse antibodies surface can bind FMC63 scFv with an affinity constant of 1.08 nM as determined in a SPR assay (Routinely tested).
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