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Your Position: Accueil > Antibody > FMC63 > CD9-M52Hb

FMC63 scFv, His Tag (MALS verified)

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  • Source
    FMC63 scFv, His Tag is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
  • Species

    Mouse

  • Conjugate

    Unconjugated

  • Antibody Type

    scFV

  • Reactivity

    Human

  • Immunogen

    CD19.

  • Specificity

    Specifically recognizes the antigen-recognition domain of CD19-derived CARs.

  • Application
    ApplicationRecommended Usage
    ELISA5-125 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FMC63 SDS-PAGE

FMC63 scFv, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
FMC63 MALS images

The purity of FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) is more than 90% and the molecular weight of this protein is around 25-37 kDa verified by SEC-MALS.

Bioactivity-ELISA
 FMC63 ELISA

Immobilized Human CD19 (20-291), Fc Tag, premium grade (Cat. No. CD9-H5251) at 5 μg/mL (100 μL/well) can bind FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) with a linear range of 0.005-0.156 μg/mL (QC tested).

  • Background
    FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.
  • Clinical and Translational Updates

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