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This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 46.5 kDa.
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mix the 10 ng /well of Monoclonal Anti-Human MSLN Antibody, Human IgG1 with the Donor PAb Anti Human IgG-Eu cryptateHuman IgG1, incubated with the Acceptor APC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H6) at increasing concentration. Detection was performed with the EC50 of 0.6644 μg/mL (Routinely tested).
5e5 of anti-MSLN CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of APC-Labeled Human Mesothelin (296-580), His Tag (Cat. No.MSN-HA2H6) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). APC signal was used to evaluate the binding activity (QC tested).
5e5 of PBMCs were stained with APC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H6) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs , and APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
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