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| ID | Components | Size |
|---|---|---|
| EP128-C01 | High-bind Plate | 1 plate |
| EP128-C02 | Human IL-5 R alpha | 30 μg |
| EP128-C03 | Human IL-5 | 25 μg |
| EP128-C04 | Human IL-5-Biotin | 10 μg |
| EP128-C05 | Streptavidin-HRP | 10 μg |
| EP128-C06 | Coating Buffer | 12 mL |
| EP128-C07 | 10xWashing Buffer | 50 mL |
| EP128-C08 | Blocking Buffer | 50 mL |
| EP128-C09 | Substrate Solution | 12 mL |
| EP128-C10 | Stop Solution | 7 mL |
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-5 R alpha.
b) Add your molecule of interest to the tests.
c) Add human IL-5-Biotin to bind the coated human IL-5 R alpha.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit IL-5: IL-5 R alpha Rbinding will be determined by comparing OD readings among different experimental groups.
![IL-5[Biotinylated]:IL-5Rα Inhibitor Screening ELISA KitIL-5[Biotinylated]:IL-5Rα Inhibitor Screening ELISA Kit (Cat. No. EP-128) ELISA bioactivity](/static/main/products/images/elisa/EP-128-E1.jpg)
INHIBITION OF IL-5 [BIOTINYLATED]: IL-5 R ALPHA BINDING BY Human IL-5
Serial dilutions of Human IL-5(Catalog # EP128-C03) (1:1 serial dilution, from 10 μg/mL to 0.020 μg/mL (666.67-0.65 nM)) was added into IL-5 R alpha: IL-5-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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